بررسی خواص مکانیکی و فیزیکی بایوکامپوزیت‌های میسلیومی قارچ Fomes fomentarius با استفاده از بسترهای لیگنوسلولزی مختلف

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه صنایع چوب و کاغذ، دانشکدة منابع طبیعی، دانشگاه زابل، زابل، ایران.

2 گروه علوم و صنایع چوب و کاغذ، دانشکدة منابع طبیعی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران.

3 گروه محیط‌ زیست، دانشکدة منابع طبیعی، دانشگاه زابل، زابل، ایران.

10.22059/jfwp.2025.392475.1342

چکیده

کامپوزیت ­های میسلیومی به ­عنوان جایگزینی سازگار با محیط­زیست، تجزیه ­پذیر و کم­هزینه برای کامپوزیت­ های سنتی، مورد توجه صنعت و پژوهش قرار گرفته ­اند. این مطالعه، با هدف تحلیل تأثیر سه بستر لیگنوسلولزی متفاوت شامل ساقة پنبه، کاه گندم و ضایعات چوب راش بر خواص فیزیکی و مکانیکی بایوکامپوزیت ­های تولیدشده با قارچFomes fomentarius  انجام شد. فرآیند تولید شامل تلقیح بسترها با میسیلیوم قارچ، انکوباسیون در شرایط کنترل­شده و پرس گرم و سرد برای ایجاد ساختار متراکم بود. آزمون­ های فیزیکی (جذب آب، واکشیدگی ضخامت) و مکانیکی (مقاومت خمشی، مدول الاستیسیتة خمشی، چسبندگی داخلی) مطابق استانداردهای EN انجام شد. نتایج نشان داد بایوکامپوزیت خرده‌ساقة پنبه با دارا بودن بالاترین ضریب ­کشیدگی (54/78) و سطح ویژة بالاتر ذرات، شبکة پیوسته­ تری از ریسه‌های قارچ تشکیل داد که منجر به ایجاد مقاومت خمشی (6/26 مگاپاسکال)، مدول الاستیسیتة خمشی (1/31 گیگاپاسکال) و چسبندگی داخلی (0/21 مگاپاسکال) مناسب شد. در مقابل، بایوکامپوزیت چوب راش به‌دلیل ضخامت بالای ذرات و حفرات ساختاری بزرگ، کمترین مقاومت مکانیکی را نشان داد. جذب آب و واکشیدگی ضخامت نیز تحت تأثیر ماهیت آبدوستی بسترها و تراکم ساختاری قرار گرفت و بایوکامپوزیت کاه گندم با جذب آب 92/77 درصدی، بالاترین و بایوکامپوزیت چوب راش با 54/44درصدی، کمترین مقدار را ثبت کردند. واکشیدگی ضخامت نیز روند مشابهی داشت. یافته ­ها تأکید می­ کنند انتخاب بستر با ویژگی‌های فیزیکی-شیمیایی مطلوب، نقش کلیدی در بهینه ­سازی عملکرد کامپوزیت­ های میسیلیومی دارد. با وجود نتایج امیدوارکننده، تحقیقات بین رشته­ ای بیشتری برای بهبود مقاومت­ ها و گسترش کاربردهای صنعتی این مواد زیست ­تخریب­ پذیر نیاز است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of mechanical and physical properties of mycelium-based biocomposites from Fomes fomentarius using different lignocellulosic substrates

نویسندگان [English]

  • Razieh Shamsi 1
  • Ali Bayatkashkoli 1
  • Saeid Reza Farukhpayam 1
  • Ali Abdolkhani 2
  • Mohsen Shahriari Moghadam 3
1 Department of Wood and Paper Industries, Faculty of Natural Resources, University of Zabol, Zabol, Iran.
2 Department of Wood and Paper Science, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
3 Department of Environmental Sciences, Faculty of Natural Resources, University of Zabol, Zabol, Iran.
چکیده [English]

Mycelium-based biocomposites have attracted significant attention from researchers and industries as sustainable alternatives to traditional composites due to their environmental compatibility, biodegradability, and low production costs. This study investigates the effects of different lignocellulosic substrates—cotton stalks, wheat straw, and beech wood waste—on the mechanical and physical properties of mycelium-based biocomposites produced with the fungus Fomes fomentarius. The production process involved substrate inoculation with fungal mycelium, incubation under controlled conditions, and hot/cold pressing to form dense structures. Physical (water absorption, thickness swelling) and mechanical tests (bending strength, bending modulus of elasticity, internal bonding strength) were conducted in accordance with EN standards. Results showed that the cotton stalk biocomposite, with the highest aspect ratio (54.78) and specific surface area of particles, formed a more continuous network of fungal hyphae, resulting in adequate bending strength (6.26 MPa), bending modulus of elasticity (1.3 GPa), and internal bonding strength (0.2 MPa). In contrast, the beech wood waste biocomposite showed the lowest mechanical performance due to high particle thickness and larger structural pores. Water absorption and thickness swelling were influenced by substrate hydrophilicity and structural density: wheat straw biocomposite had the highest water absorption (77.92%), while beech wood waste had the lowest (54.44%). This study highlights that selecting substrates with optimal physicochemical properties is crucial for optimizing the performance of mycelium-based composites. However, further interdisciplinary research is needed to enhance resistance and expand industrial applications of these biodegradable materials.

کلیدواژه‌ها [English]

  • Fungal mycelium
  • Fomes fomentarius
  • Lignocellulosic substrate
  • Mechanical and physical properties
  • Mycelium biocomposite
[1] Sydor, M., Bonenberg, A., Doczekalska, B. & Cofta, G. (2021). Mycelium-based composites in art, architecture, and interior design: a review. Polymers, 14(1), 145-159.
[2] Van Empelen, J.C. (2018). A Study into More Sustainable, Alternative Building Materials as A Substitute for Concrete in Tropical Climates. Delft University of Technology, Delft, Netherlands, 1-26.
[3] Shao, G., Zhang, L., Xu, D., Jin, Y., Wu, F., Yang, N. & Xu, X. (2025). Green and sustainable bioboards: Biomanufacturing of mycelium-based composite boards with tunable properties. Chemical Engineering Journal, 503, 158382.
[4] Liu, R., Li, X., Long, L., Sheng, Y., Xu, J. & Wang, Y. (2020). Improvement of mechanical properties of mycelium/cotton stalk composites by water immersion. Composite Interfaces, 27(10), 953-966.
[5] Seethapathy, P., Subramani, T., Ayrilmis, N. & Patil, H. (2025). Therapeutic and Environmental Potential of Mushrooms in Ancient and Modern Contexts: A Review. International Journal of Medicinal Mushrooms, 27, 1-19.
[6] Gow, N. A., Latge, J.P. & Munro, C.A. (2017). The fungal cell wall: structure, biosynthesis, and function. Microbiology Spectrum, 5(3), 10-1128.
[7] Van den Brandhof, J.G. & Wösten, H.A. 2022. Risk assessment of fungal materials. Fungal biology and Biotechnology, 9(1), 3-12.
[8] Větrovský, T., Voříšková, J., Šnajdr, J., Gabriel, J. & Baldrian, P. (2011). Ecology of coarse wood decomposition by the saprotrophic fungus Fomes fomentarius. Biodegradation, 22, 709-718.
[9] Zhang, M., Xue, J., Zhang, R., Zhang, W., Peng, Y., Wang, M., & Cao, J. (2023). Mycelium composite with hierarchical porous structure for thermal management. Small, 19(46), 2302827.
[10] De Paula, R.G., Antoniêto, A.C.C., Ribeiro, L.F.C., Srivastava, N., O'Donovan, A., Mishra, P.K., Gupta, V.K. & Silva, R.N. (2019). Engineered microbial host selection for value-added bioproducts from lignocellulose. Biotechnology Advances, 37(6), 107347.
[11] Lustenhouwer, N., Maynard, D.S., Bradford, M.A., Lindner, D.L., Oberle, B., Zanne, A.E. & Crowther, T.W. (2020). A trait-based understanding of wood decomposition by fungi. Proceedings of the National Academy of Sciences, 117(21), 11551-11558.
[12] Shin, H. J., Ro, H. S., Kawauchi, M. & Honda, Y. (2025). Review on mushroom mycelium-based products and their production process: from upstream to downstream. Bioresources and Bioprocessing, 12(1), 1-21.
[13] Heisel, F., Lee, J., Schlesier, K., Rippmann, M., Saeidi, N., Javadian, A., Nugroho, A.R., Van Mele, T., Block, P. & Hebel, D.E. (2017). Design, cultivation and application of load-bearing mycelium components: The MycoTree at the 2017 Seoul Biennale of architecture and urbanism. International Journal of Sustainable Energy, 6(1), 296-303.
[14] Voutetaki, M.E. & Mpalaskas, A.C. (2024). Natural fiber-reinforced mycelium composite for innovative and sustainable construction materials. Fibers, 12(7), 57-69.
[15] Sun, W., Tajvidi, M., Hunt, C.G., Cole, B.J., Howell, C., Gardner, D.J. & Wang, J. (2022). Fungal and enzymatic pretreatments in hot-pressed lignocellulosic bio-composites: A critical review. Journal of Cleaner Production, 353, 131659.
[16] Balaeș, T., Radu, B.M. & Tănase, C. (2023). Mycelium-composite materials—a promising alternative to plastics?. Journal of Fungi, 9(2), 210-219.
[17] Womer, S., Huynh, T. & John, S. (2023). Hybridizations and reinforcements in mycelium composites: A review. Bioresource Technology Reports, 101456.
[18] Doğan, N. & Doğan, C. (2022). Development of the non-grain spawn for edible mushroom (Pleurotus ostreatus): D-optimal mixture design approach. Ege Üniversitesi Ziraat Fakültesi Dergisi, 59(2), 265-274.
[19] Jones, M., Mautner, A., Luenco, S., Bismarck, A. & John, S. (2020). Engineered mycelium composite construction materials from fungal biorefineries: A critical review. Materials and Design, 187, 108397.
[20] Girometta, C., Picco, A.M., Baiguera, R.M., Dondi, D., Babbini, S., Cartabia, M., Pellegrini, M. & Savino, E. (2019). Physico-mechanical and thermodynamic properties of mycelium-based biocomposites: A review. Sustainability, 11(1), 281-292.
[21] Arabi, M., Haftkhani, A.R. & Pourbaba, R. (2023). Investigating the effect of particle slenderness ratio on optimizing the mechanical properties of particleboard using the response surface method. BioResources, 18(2), 2800-2814.
[22] Bagheriehnajjar, G., Yousefpour, H. & Rahimnejad, M. (2023). Multi-objective optimization of mycelium-based bio-composites based on mechanical and environmental considerations. Construction and Building Materials, 407, 133346.
[23] Attias, N., Danai, O., Tarazi, E., Pereman, I. & Grobman, Y. J. (2019). Implementing bio-design tools to develop mycelium-based products. The Design Journal, 22(sup1), 1647-1657.
[24] Kraisornkachit, P., Naito, M., Kang, C. & Sato, C. (2024). Multi-Objective Optimization of Adhesive Joint Strength and Elastic Modulus of Adhesive Epoxy with Active Learning. Materials, 17(12), 2866-2875.
[25] Manan, S., Ullah, M.W., Ul-Islam, M., Atta, O.M. & Yang, G. (2021). Synthesis and applications of fungal mycelium-based advanced functional materials. Journal of Bioresources and Bioproducts, 6, 1-10.
[26] Rigobello, A. & Ayres, P. (2023). Design strategies for mycelium-based composites. In Fungi and Fungal Products in Human Welfare and Biotechnology (pp. 605-635). Singapore: Springer Nature Singapore.
[27] Somal, M., Chauhan, S.S. & Kumar, R. (2024). Mycelium-Based Lignocellulosic Composites: Sustainable Innovations in Green Materials. In Encyclopedia of Green Materials (pp. 1-9). Singapore: Springer Nature Singapore.
[28] Jin, Y., De, G., Wilson, N., Qin, Z. & Dong, B. (2025). Towards Carbon-Neutral Built Environment: A Critical Review of Mycelium-Based Composites. Energy and Built Environment.
[29] Voutetaki, M. E., & Mpalaskas, A. C. (2024). Natural fiber-reinforced mycelium composite for innovative and sustainable construction materials. Fibers, 12(7), 57-69.
[30] Liu, R., Long, L., Sheng, Y., Xu, J.F., Qiu, H.Y., Li, X.Y., Wang, Y.X. & Wu, H.G. (2019). Preparation of a kind of novel sustainable mycelium/cotton stalk composites and effects of pressing temperature on the properties. Industrial Crops and Products, 141, 111732.
[31] Aiduang, W., Kumla, J., Srinuanpan, S., Thamjaree, W., Lumyong, S. & Suwannarach, N. (2022). Mechanical, physical, and chemical properties of mycelium-based composites produced from various lignocellulosic residues and fungal species. Journal of Fungi, 8(11), 1125-1132.
[32] Weiland, K., Jones, M. P., Zinsser, F., Kontturi, E., Mautner, A. & Bismarck, A. (2021). Grow it yourself composites: Delignification and hybridisation of lignocellulosic material using animals and fungi. Green Chemistry, 23(19), 7506-7514.
[33] Cheng, Y., Long, C., Zhang, M., Wang, W. & Sun, W. (2024). Investigation of mycelium film as the adhesive for poplar veneer bonding: insight into interfacial bonding mechanisms. Wood Material Science & Engineering, 1-10.
[34] Gezer, E.D. & Kuştaş, S. (2024). Acoustic and thermal properties of mycelium-based insulation materials produced from desilicated wheat straw–Part B. BioResources, 19(1), 1348.
[35] Girometta, C., Dondi, D., Baiguera, R. M., Bracco, F., Branciforti, D.S., Buratti, S., Lazzaroni, S. & Savino, E. (2020). Characterization of mycelia from wood-decay species by TGA and IR spectroscopy. Cellulose, 27, 6133-6148.
[36] Kalpokaitė-Dičkuvienė, R., Pitak, I., Sholokhova, A., Kriūkienė, R. & Baltušnikas, A. (2024). Surface-modified wheat straw for the production of cement-free geopolymer composite: effects of wheat variety and pre-treatment method. Journal of Composites Science, 8(4), 116-124.
[37] Elsacker, E., Vandelook, S., Brancart, J., Peeters, E. & De Laet, L. (2019). Mechanical, physical and chemical characterisation of mycelium-based composites with different types of lignocellulosic substrates. PLoS One, 14(7), e0213954.
[38] Peng, L., Yi, J., Yang, X., Xie, J. & Chen, C. (2023). Development and characterization of mycelium bio-composites by utilization of different agricultural residual byproducts. Journal of Bioresources and Bioproducts, 8(1), 78-89.
[39] Sun, F.F., Wang, L., Hong, J., Ren, J., Du, F., Hu, J., Zhang, Z. & Zhou, B. (2015). The impact of glycerol organosolv pretreatment on the chemistry and enzymatic hydrolyzability of wheat straw. Bioresource Technology, 187, 354-361.
[40] Chulikavit, N., Huynh, T., Khatibi, A., Das, R. & Kandare, E. (2023). Thermal degradation and flame spread characteristics of epoxy polymer composites incorporating mycelium. Scientific Reports, 13(1), 17812.
[41] Sisti, L., Gioia, C., Totaro, G., Verstichel, S., Cartabia, M., Camere, S. & Celli, A. (2021). Valorization of wheat bran agro-industrial byproduct as an upgrading filler for mycelium-based composite materials. Industrial Crops & Products, 170, 113742.
[42] Pena, R., Lang, C., Naumann, A. & Polle, A. (2014). Ectomycorrhizal identification in environmental samples of tree roots by Fourier-transform infrared (FTIR) spectroscopy. Frontiers in Plant Science, 5, 229.
[43] Răut, I., Călin, M., Vuluga, Z., Oancea, F., Paceagiu, J., Radu, N., Doni, M., Alexandrescu, E., Purcar, V., Gurban, A.M. & Petre, I. (2021). Fungal based biopolymer composites for construction materials. Materials, 14(11), 2906-2919.