Investigating the effects of growth regulators benzyladenine, thidiazuron and gibberellic acid on the regeneration of chestnut (Castanea sativa Mill.)

Document Type : Research Paper

Authors

1 Department of Forestry, Faculty of Natural Resources, Guilan University, Sowmeh Sara, Iran.

2 Faculty of Tissue Culture and Molecular Biology, Agricultural Biotechnology Research Institute of North Region, Rasht, Iran.

10.22059/jfwp.2024.370173.1274

Abstract

Chestnut (Castanea sativa Mill.) is one of the native species of Guilan province. Due to fungal diseases and animal grazing, as well as the high amount of unhealthy seeds, native chestnut in vitro culture is one of the effective methods for mass production of this endangered plant. The aim of this research is to determine the optimal culture medium for chestnut tree regeneration through tissue culture. The culture medium used in this research includes GD (Gresshoff & Doy), DKW (Driver and Kuniyuki Walnut) (1 mg/liter BA*) and (Murashige Skoog) MS (half nitrogen concentration) with three different hormone combinations (1 mg/liter + BA 0.1 mg per liter TDZ), (0.5 mg/liter TDZ), and (1 mg/liter + GA3 0.1 mg/liter BA). For in vitro culture of chestnut, lateral buds of one-year-old plants grown in the greenhouse were used. One-way analysis of variance (ANOVA) was performed to investigate the effect of culture media with plant growth regulators on explant length, number of leaves, and stems. The results showed that the length of explants has a significant difference in culture media. No significant difference was observed in the number of stems and leaves. The results of the comparison of means using Tukey's test showed a significant difference between the length of explants in DKW medium and other media. DKW medium with 1 mg/liter BA was found to be the best medium for regeneration and longitudinal growth of chestnut explants. In the present study, in vitro culture of Chestnut is introduced an effective method for mass production of this species.

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References

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Forest and Wood Products
Volume 77, Issue 1
June 2024
Pages 47-54

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