The Effect of Growth-Promoting Rhizobacteria on Morphological Growth Indices of Cerasus mahaleb (L.) Mill's Provenances - (Case study: Fereydunshahr)

Document Type : Research Paper

Authors

1 Ph.D. Forestry and Forest Ecology, Sari Agricultural Sciences and Natural Resources University, Sari, I.R. Iran.

2 Assist., Prof., Division of Natural Resources, Isfahan Agricultural and Natural Resources, Agricultural, Research, Education and Extension Organization, Isfahan, I.R. Iran.

10.22059/jfwp.2022.337203.1203

Abstract

In order to investigate the effect of inoculation of Cerasus mahaleb provenances in Fereydunshahr city of Isfahan with the most important rhizosphere bacteria stimulating plant growth on morphological indices of Cerasus mahaleb, a factorial experiment was planned in a completely randomized block design with three replications in a greenhouse. Inoculation of seedling with five levels including non-bacterial inoculation (as a control), inoculation with bacteria (Bacillus sp.), (Azotobacter sp.), (Pseudomonas fluorescens) and combination treatment of three growth-promoting bacteria (MIX) and calculation of characteristics morphological analysis of Cerasus mahaleb was performed. In the study of morphological traits of seedlings, combined treatment (MIX) of bacteria showed the best performance in stem length (27.66 cm), root length (70.61 cm), basal diameter (9.07 mm), shoot (21.62 g) and root (28.45 g) dry weight, and number of leaves (22). Only in leaf area (23.82 cm), bacterial treatment (Pseudomonas fluorescens) had better performance than MIX. The results showed that the weakest performance among bacterial growth stimulant treatments was related to bacteria (Bacillus sp.). Among the 10 study areas, Chal Khalil 1 and 2 forest reserve areas as well as Poshtkuh Durak 2 showed the best morphological traits compared with other areas in bacterial inoculation. In fact, inoculation of seedlings with growth-promoting bacteria can be a good way to produce healthy and strong seedlings as well as better establishment and increase the success of seedlings in disturbed and degraded habitats of this important species of Zagros forests.

Keywords


[1]. Sabeti, H., (1976). Forests, Trees and Shrubs of Iran. Yazd University Press, Yazd, 810p.
[2]. Zanganeh, H. (1999). Report of existence Cerasus mahaleb (L.) Mill. in Kermanshah province forests. Published by Forests, Range and Watershed Management Organization, Tehran, 13p.
[3]. Khatam Saz, M. )1992(. Flora of Iran, No. 6: Rosaceae, Publications of the Forest and Rangeland Research Institute. First Edition. 352 p.
[4]. Modarres Hashemi, M., Panahpour, H., and Ismaili Sharif, M. (2010). National plan for collection, identification, maintenance, evaluation, reproduction and application of genetic resources of forest trees and shrubs in Iran (Isfahan province). Final report of the research project, Publications of the Forest and Rangeland Research Institute. 59 Pp.
[5]. Shahryari, H., Rostami Shahraji, T., Sayad, E., and Yousef Nanaei, S. (2012). Investigation of some ecological condition of Cerasus mahaleb Miller (Rock cherry sp.) in Khouzestan province (county baghmalek forests). Iranian Journal of Forest and Poplar Research, 20(1): 137-150.
[6]. Mafia, R.G., Alfenas, A.C., Ferreira, E.M., Henrique, D., Binoti, B., Mafia, G.M.V., and Moun-teer, A.H., (2009). Root   colonization   and   interaction   among growth promoting rhizobacteria isolates and eucalyptsspecies. Revista Árvore, 33 (1): 1–9.
[7]. Karakurt, H., and Aslantas, R. (2010). Effects of some plant growth promoting rhizobacteria (PGPR) strains on plant growth and leaf nutrient content of apple. Fruit Ornamental Plant Resources, 18: 101-110.
[8]. Erturk, Y., Ramasan, E., Omur, D., and Metin, T. (2011). Effects of plant growth promotion rhizobacteria on vegetative growth and leaf nutrient content of hazelnut seedling (Tomul and Sivri). International Journal of Soil Science, 42: 91-98.
[9]. Karličid, V., Radid, D., Jovičid Petrovid, J., Golubovid-Durguz, V., Kikovid, D., and Raičevid, V. (2015). Inoculation of Robinia pseudoacacia L. and Pinus sylvestris L. seedlings with plant growth promoting bacteria causes increased growth in coal mine overburden. In: Ivetid, V., and Stankovid, D. (Eds.). Proceedings of International Conference on Reforestation Challenges. Belgrade, Serbia, Reforesta, 3-6 June. 42-49.
[10]. Teimouri, M., Korori, S.A.A., Khoshnevis, M., Matinizadeh, M., and Kalagari, M. (2007). Effect of phosphate solubilizing bacteria inoculation on maple (Acer velutinum) seedlings. Iranian Journal of Forest and Poplar Research, 15(1): 76-82.
[11]. Hasani, G., Akhgar, A.A.R., and Tajabadpour, A. (2012). Effectiveness of IAA and ACCDeaminaze producing Pseudomonas fluorescent on Growth of Pistachio seedling. Iranian Journal of Soil Research, 26(1): 89-97.
[12]. Bahmani, M., Jalali, G.A., Asgharzadeh, A., and Tabari, M. (2015). Efficiency of Pseudomonus putida 169 on improvement few growth characters of Calotropis procera seedling under drought stress. Soil Biology, 2(3): 107-116.
[13]. Yang, Y., Liu, Q., Han, C., Qiao, Y.Z., Yao, X.Q., and Yin, H.J. (2007). Influence of water stress and low irradiance on morphological and physiological characteristics of Picea asperata seedlings. Photosynthetica, 45: 613-619.
[14]. Basra, S. M. A., Ashraf, M., Iqbal, N., Khaliq, A., and Ahmad, R. (2004). Physiological and biochemical aspects of pre- sowing heat stress on cotton seed. Seed Science and Technology, 32:765- 774.
[15]. Hojjat Noughi, F., Akhgar, A.R., Esfandiarpour, I., and Khavazi, K. (2013). Evaluation of population and properties of PGPB of Endorhizosphere, Rhizosphere and Nonrhizosphere in Pistachio seedlings. Iranian Journal of Soil and Water Sciences, 23(4): 215- 234.
[16]. Stepanova, A.N., Robertson-Hoyt, J., Yun, L.M., Benavente, D.Y., Xie, K., Dolezal, S., Jurgens, G., and Alonso, J.M. (2008). TAA1-mediated Auxin biosynthesis is essential for hormone crosstalk and plant development. Cell, 133: 177-191.
[17]. Esitken, A., Yildiz, H.E., Ercisli, S., Donmez, M.F., Turan, M., and Gunes, A. (2010). Effects of plant growth promoting bacteria (PGPB) on yield, growth and nutrient contents of organically grown strawberry. Scientia Horticulturae, 124: 62-66.
[18]. Rekha, P.D, Lai,W.A.,  Arun,A.B.,  Young,C.C. (2007).  Effect of free and encapsulated Pseudomonas putida CC-FR2-4 and Bacillus subtilisCC-pg104 on plant growth under gnotobiotic condition. Bio Resour Tech, 98:447–451.
[19]. Patel, D., and Saraf, M. (2013). Influence of soil ameliorants and microflora on induction of antioxidant enzymes and growth promotion of Jatropha curcas L. under saline condition. European Journal of Soil Biology, 55:47-54.
[20]. Lata Nain, M., Yadav, R.C., and Saxena, J. (2012). Characterization of multifaceted Bacillus sp. RM-2 for its use as plant growth promoting bioinoculant for crops grown in semi-arid deserts. Applied Soil Ecology 59:124– 135.
[21]. Rostamikia, Y., Tabarei Koochaksaraei, M., Asgharzade, A. and Rahmani, A. (2017). Effect of Growth Promoting Rhizobacteria on growth and nutrient elements of common hazelnut (Corylus avellana L.) seedlings in Ardabil Fandoqlou nursery. Iranian Journal of Forest and Poplar Research. 25(1): 116-126.