Effect of inoculation of two mycorrhizal fungi and two growth-promoting rhizobacteria on improvement of characteristics of Myrtus Communis L. seedlings under drought stress

Document Type : Research Paper

Authors

1 Ph.D. Student of Silviculture, Faculty of Natural Resources, Tarbiat Modares University, Noor, I.R. Iran

2 Prof., Department of Forestry, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, I.R. Iran

3 Assoc. Prof., Soil and Water Research Institute, Agricultural Research Education and Extension Organization (AREEO) Karaj, I.R. Iran

4 Prof., Faculty of Agriculture, Tarbiat Modares University, Tehran, I.R. Iran

Abstract

The present study was carried out to investigate the effect of two mycorrhizal fungi and two growth-promoting rhizobacteria on survival and growth traits of Myrtus communis L. in water deficit conditions, as a completely randomized factorial design. Drought stress in three levels: 100% field capacity (without stress), 60% field capacity (mild stress) and 30% field capacity (severe stress) and biofertilizer in seven levels: control (without inoculation); inoculation with mycorrhizals of Funneliformis mosseae, Rhizophagus intraradices, and combination of these two fungi; inoculation with rhizobacterias of Pseudomonas fluorescens, P. putida, and combination of these two bacteria was considered in three replicates. Drought stress reduced and biofertilizers (in particular, composition of fungus, and combination of bacterias) increased the studied traits. In severe water deficit, combined treatments of fungal or bacterial improved height increment by 28-31%, leaf biomass by 51-52%, root biomass by 36-42%, total biomass by 37-41% and survival by 50% compared with the control (non-inoculation) ones. In severe drought stress, almost 50% of the seedlings survived without inoculation and 90-100% of them with inoculation, indicating the high tolerance of this species to drought. Due to the reduction of the destructive effect of water stress on M. communis seedling traits using biofertilizers, the use of these treatments, especially the combination of two fungi of Funneliformis mosseae and Rhizophagus intraradices or two rhizobacteria of Pseudomonas fluorescens and P. putida can be useful to improve seedling's growth characteristics.

Keywords

References

[1]. Trenberth, K.E., Dai, A., Van Der Schrier, G., Jones, P.D., Barichivich, J., Briffa, K.R., and Sheffield, J. (2014). Global warming and changes in drought. Nature Climate Change, 4 (1):17–22.
[2]. Anjum, Sh. A., Xie, X.Y., Wang, Ch., Saleem, M.F., Man, Ch., and Lei, W. (2011). Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research. 6(9): 2026-2032.
[3]. Yin, N., Zhang, Z., Wang, L., and Qian, K. (2016). Variations in organic carbon, aggregation, and enzyme activities of gangue-fly ash-reconstructed soils with sludge and arbuscular mycorrhizal fungi during 6-year reclamation. Environmetal Science Pollution Research, 23(17):17840–17849.
[4]. Henry, S., Texier, S., Hallet, S., Bru, D., Dambreville, C., Chèneby, D., Bizouard, F., Germon, J.C., and Philippot, L. (2008). Disentangling the rhizosphere effect on nitrate reducers and denitrifiers: insight into the role of root exudates. Environmental Microbiology, 10(11): 3082–3092.
[5]. Bahmani, M., Jalali, GH. A., Asgharzadeh, A., and Tabari Kochaksaraeai, M. (2015). Efficiency of rhisobacter Pseudomonus putida 139 inoculation on the improvement of some growth traits of Carotropis procera Ait. seedlings under drought stress. Irainan Journal of Soil Biology, 3 (2): 102-116.
[6]. Irankhah, S., Ganjali, A., Lahooti, M., and Mashreqi, M. (2016). Effect of Pseudomonas putida and Glomus intraradices on some morphological and biochemical traits of Trigonella foenum-graecum (L). Irainan Journal of Horticultural Science, 30 (1): 112-121.
[7]. Gong, M., You, X., and Zhang, Q. (2015). Effects of Glomus intraradices on the growth and reactive oxygen metabolism of foxtail millet under drought. Annals of Microbiology, 65(1): 595-602.
[8]. Amiri, R., Nikbakht, A., Rahimmalek, M., and Hosseini, H. (2017). Variation in the essential oil composition, antioxidant capacity, and physiological characteristics of Pelargonium graveolens L. inoculated with two species of mycorrhizal fungi under water deficit conditions. Journal of Plant Growth Regulation, 58 (1): 1-14.
[9]. Amiri, N., Emadian, S.F., Fallah, A., Adeli, K., and Amirnejad, H. (2016). Estimation of conservation value of myrtle (Myrtus communis) using a contingent valuation method: a case study in a Dooreh forest area, Lorestan Province, Iran .Forest Ecosystems, 14(1): 26-36.
[10]. Zarik, L., Meddich, A., Hijri, M., Hafidi, M., Ouhammou, A., Ouahmane, L., Duponnois, R., and Boumezzough, A. (2016). Use of arbuscular mycorrhizal fungi to improve the drought tolerance of Cupressus atlantica G. Comptes Rendus Biologies, 339 (5-6): 185–196.
[11]. Yin, Ch., Pang, X., and Chen, K. (2009). The effects of water, nutrient availability and their interaction on the growth, morphology and physiology of two poplar species. Environmental and Experimental Botany, 67 (1): 196-203.
[12]. Khalvati, M.A., Mzafar, A., and Schmidhalter, U. (2005). Quantification of water uptake by arbuscular mycorrhizal hypha and its signification for leaf growth, water relations and gas exchange of barley subjected to drought stress. Plant Biology Stuttgart, 7(6): 706-712.
[13]. Marulanda, A., Barea, J.M., and Azcon, R. (2009). Stimulation of plant growth and drought tolerance by native microorganisms (AM fungi and bacteria) from dry environments: mechanisms related to bacterial effectiveness. Journal of Plant Growth Regulation, 28 (2): 115-124.
[14]. Caoa, X., Chen, Ch., Zhang, D., Shu, B., Xiao, J., and Xia, R. (2013). Influence of nutrient deficiency on root architecture and root hair morphology of trifoliate orange. Scientia Horticulturae, 162 (1): 100–105.
[15]. Garg, N., and Bhandari, P. (2016). Silicon nutrition and mycorrhizal inoculations improve growth, nutrient status, K+/Na+ ratio and yield of Cicer arietinum L. genotypes under salinity stress. Plant Growth Regulation, 78(3):371–387.
[16]. Shaharoona, B., Naveed, M., Arshad, M., and Zahir, Z.A. (2008). Ferttilizer-dependent efficiency of Pseudomonas for improving growth, yield and nutrient use efficiency of wheat (Triticum aestivum L.). Microbiol Biotechnology, 79 (1): 147-155.
[17]. James, B., Rodel, D., Lorettu, U., Reynaldo, E., and Tariq, H. (2008). Effect of vesicular arboscular mycorrhiza (VAM) fungi inoculation on coppicing ability and drought resistance of Senna Fspectabilis. Pakistan Journal of Botany, 40 (5): 2217-2224.
[18]. Khalid, K.A. (2006). Influence of water stress on growth, essential oil and chemical composition of herbs (Ocimum sp.). International Agrophysics 20 (1): 289- 296.
[19]. Ahemad, M., Kibret, M. (2014). Mechanisms and applications of plant growth promoting Rhizobacteria: Current perspective. Journal of King Saud University-Science, 26 (1): 1-20.
Forest and Wood Products
Volume 73, Issue 3
November 2020
Pages 271-281

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