Studying the seed germination traits of Seabuckthorn (Elaeagnus rhamnoides) of Iran, China and Tibet

Document Type : Research Paper

Authors

Abstract

This study was conducted for the first time in order to introduce the pioneer forest trees and medicinal sea-buckthorn (Elaeagnus rhamnoides) and determine the characteristics of the species seeds from seven provenances, including Iran, China and Tibet in the laboratory. After site identification of the species, seeds were collected from the regions including Alborz, Mazandaran, Eastern Azerbaijan, Western Azerbaijan, Qazvin, China and Tibet. The disinfected petri dishes were then placed into a germinator. The seeds were planted as completely randomized design in four replicates each with 25 seeds. At the end of seed growth periods, the traits including germination percentage, number of grown seeds, mean of germination time, germination rate, germination energy, seed vigor index, sum of plumule length, sum of radicle length, mean of plumule length, mean of radicle length, allometry, plumule number, radicles number and the ratio of radicles to plumule number were measured and/ or calculated. Averages of germination percentage were 90, 48, 95, 36, 33, 32 and 60 for Alborz, Mazandaran, Eastern Azerbaijan, Western Azerbaijan, Qazvin, China and Tibet, respectively. The maximum and minimum germination percentages were observed for Eastern Azerbaijan and China provenances, respectively. These indices were 54.1 and 20.6 for Eastern Azerbaijan and China, respectively. Allometry which is the ratio of the radicle length to the plumule length was maximum in Mazandaran with 2.4 and Qazvin with 0.95. This study showed that Seabuckthorn propagation by seeds is feasible and recommendable. Further researches are recommended on this valuable species due to its drought tolerance, nitrogen fixing ability and unique medicinal properties

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[2]. Ranal, M.A., and Santann, D.G. (2006). How and why to measure the germination process? Revista
Brasileira de Botanica, 29(1): 1-11.
[3]. Marvi-Mohadjer, M.R. (2006). Silviculture. University of Tehran Press, 387 p.
[4]. Sabeti, H. (1976). Forests, trees, and shrubs of Iran, University of Yazd Press, 1009 p.
[5]. Mozaffarian, V. (2005). Trees and Shrubs of Iran. Farhang Moaser Publishers. 1120 p.
[6]. Zhang, H.Q., Tang, M., Chen, H., Tian, Z.Q., Xue, Y.Q., and Feng, Y., (2010). Communities of arbuscular mycorrhizal fungi and bacteria in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed. Plant and Soil, 326: 415–424.
[7]. Geetha, S., and Asheesh, G. (2011). Review Medicinal and therapeutic potential of Sea buckthorn (Hippophae rhamnoides L). Journal of Ethnopharmacology, 138: 268– 278.
[8]. Lu, R., and Ahani, H. (2013). The Genetic Resources of Hippophae genus and its utilization. Advance in Agriculture and Biology, 1 (2), 2013: 27-31.
[9]. Lu, R., (1992). Seabuckthorn. A multipurpose plant species for fragile mountains. Katmandu, Nepal: ICIMOD Publication unit. 47 p.
[10]. Geetha S., Sai Ram M., Singh V., Ilavazhagan, G., and Sawhney, RC. (2002). Anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides) an in vitro study. Journal of Ethnopharmacology, 79(3):373-378.
[11]. Michel, T., Destandau, E., Floch, G., Elisabeth Lucchesi, M., and Elfakir C. (2012). Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn (Hippophaë rhamnoides L.) leaf, stem, root and seed. Food Chemistry, 131: 754–760.
[12]. Vilela, A.E., and Ravetta, D.A. (2001). The effect of seed scarification and soil-media on germination, growth, storage, and survival of seedlings of five species of Prosopis L.(Mimosaceae). Journal of Arid Environments, 48: 171-184.
[13]. Kuriakose, S.V., and Prasad, M.N.V. (2008). Cadmium stress affects seed germination and seedling growth in Sorghum bicolor (L.) Moench by changing the activities of hydrolyzing enzymes. An International Journal on Plant Growth and Development, 54(2): 143-156.
[14]. Ahani H., Jalilvand H., Vaezi J. and Sadati S.E. (2014). Investigation of nursery treatments on sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) seed germination in the field. Journal of Biodiversity and Environmental Sciences, 4 (6): 8-18.
[15]. Li T.S.C., and Wardle D.A. (1999). Effects of Seed Treatments and Planting Depth on Emergence of Sea Buckthorn Species. Horthechnology, 9(2), 213-216.
[16]. Korekar G., Dwivedi S.K., Singh H., Srivastava R.B., and Stobdan T. (2013). Germination of Hippophae rhamnoides L. seed after 10 years of storage at ambient condition in cold arid. Research Communication: Current Science, 104 (01), 110-114.
[17]. Vashistha R.K., Chaturvedi A.K., Gairola S., and Nautiyal M.C. (2013). Seed germination improvement in Elaeagnus rhamnoides (L.) A. Nelson (Sea Buckthorn) by Gibberellic acid treatment. International Journal of Medicinal and Aromatic Plants, 3(3): 382-385
[18]. Gholami, P., Ghorbani, J., Ghaderi, SH.,Salarian, F., and Karimzadeh, A. (2010). Assessment of germination indices Vicia monantha under salinity and drought stresses. Rangeland, 4(1):1-11.
[19]. Panwar, P., and Bhardwaj, S.D. (2005). Handbook of practical forestry, Agrobios (INDIA), 191p.
[20]. Kulkarni, M.G., Street, R.A., and Staden J.V., (2007). Germination and seedling growth requirements for propagation of Diosscorea dregeana (Kunth) et Schinz-A tuberous medicinal plant. South African Journal of Botany, 33: 131-137.
[21]. Mesdaghi, M. (2012). Methods of statistic and regression, Emam Reza University Press. P.421.
[23]. Pederson, L., Jqrgensen, P.E. and Poulsen, I. (1993). Effect of seed vigor and dormancy on field emergence, development and grain yield of winter wheat (Triticum aestivum L). Journal seed science Technology, 21: 159-178.
[24]. Bishnoi, N.R., Sheroran, I.S., and Singh, R. (1993). Effect of cadmium and nickel on mobilization of food reserves and activities of hydrolytic enzymes in germinating pigeon pea seeds. Biology Plant, 35:583–589.
[25]. Zeinali, E., Soltani, A., Galeshi, S., and Sadati, J. (2011). Cardinal temperatures, response to temperature and range of thermal tolerance for seed germination in wheat (Triticum aestivum L) cultivars.Electronic Journal of Crop Production, 3(3): 23-42.
[26]. Soltanipoor, M.A., Asadpoor, R., Hajebi, A., and Moradi, N. (2010). Study of pre treatments on seed germination of Foeniculum vulgare L., Salvia sharifii Rech. et Esfand and Abutilon fruticosum Guill et Perr. Iranian journal of medicinal and aromatic plants, 25(4 (46)):529-539.