In this research, effects of pre-steaming of fir wood (Abies alba) on the ACC preservative absorption, retention, and depth of penetration were investigated. Pre-steaming was carried out at 120, 140, and 160°C for one hour under 2 to 3 bars pressure. Steaming was carried out for bastard sawn boards with green dimensions of 250 × 100 × 50 millimeter and average initial moisture content of 30%. After moisture conditioning of wood samples, they were impregnated with ACC preservative solution with 5% concentration using low pressure process. Then, the impregnation properties were measured. The image J software was also used to determine the wood cross section impregnation. Results showed that pre-steaming at 140 and 160°C significantly increased the absorption, retention, and depth of preservative penetration; however, no significant difference was observed between the control specimens with those pre-steamed at 120°C. Furthermore, the results showed that the wood impregnation properties, including absorption and depth of penetration improved by increasing steaming temperature from 120 to 160⁰ C.