ارزیابی میزان فنل و فلاونوئید تام و فعالیت آنتی‏اکسیدانی پوست درختان راش، ممرز، و صنوبر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد صنایع خمیر و کاغذ دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 استادیار گروه صنایع چوب و کاغذ دانشگاه علوم کشاورزی و منابع طبیعی ساری

3 دانشیار گروه شیمی دارویی دانشگاه علوم پزشکی ساری

چکیده

هدف این مطالعه، بررسی میزان فنول و فلاونوئید تام و بررسی اثر آنتی‏اکسیدانی پوست درختان راش، ممرز، و صنوبر است. راش و ممرز از گونه‌‌‌‌‌‌های جنگلی شمال ایران، و صنوبر از گونه‌‌‌‌‌‌های دست‏کاشت است، ولی برخلاف فراوانی این درختان، اطلاعات بسیار کمی در مورد ترکیبات شیمیایی پوست آن‌ها و کاربردشان ارائه شد‌‌‌‌ه است. ترکیبات آنتی‏اکسیدانی که از شیوع بیماری‌‌‌‌‌‌‌های مزمن و تخریب بسیاری از مواد غذایی جلوگیری می‏کنند، از پوست این گیا‌‌‌‌هان نیز قابل استخراج است. بدین‌سبب، عصارۀ استخراج‌شده از گیا‌‌‌‌هان، که باعث کاهش شیوع بیماری‏‌‌‌‌‌‌های مزمن می‏شود، در صنایع دارویی بسیار اهمیت دارد. پس از تهیة پوست این درختان، عصاره‏‌‌‌‌‌‌های استنی به روش سوکسوله استخراج شدند. ابتدا میزان فنول و فلاونوئید عصاره‌‌‌‌ها اندازه‏گیری و سپس برای ارزیابی خواص آنتی‏اکسیدانی عصاره‌‌‌‌‌‌های استخراج‌‌شده، از دو روش دی ‌‌فنیل‌‌ پیکریل ‌‌هیدرازیل و قدرت احیاکنندگی، استفاده شد. نتایج نشان داد که میزان فنول و فلاونوئید در پوست درخت ممرز بیشترین، و در راش کمترین مقدار بود‌‌‌‌ه است. نتایج آزمون به‌دام‏اندازی رادیکال‌‌‌‌‌‌‌های آزاد دی‌‌ فنیل‌‌ پیکریل‌‌ هیدرازیل نشان داد که غلظت مهار 50 درصد در عصارة استنی پوست درختان راش، ممرز، و صنوبر به‌ترتیب 07/63، 17/33، و 3/86 میکروگرم بر میلی‏لیتر بود‌‌‌‌ه است. همچنین عصارة راش قدرت احیاکنندگی بیشتری در مقایسه با سایر گونه‌‌‌‌‌‌ها داشت.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of the Antioxidant Capacities and Total Phenolic Contents of Beech, Hornbeam and Poplar Barks

نویسندگان [English]

  • Roya Fazli 1
  • Nooredin Nazarnezhad 2
  • Mohamadali Ebrahimzadeh 3
1 M.Sc. Student, University of Agricultural Sciences and Natural Resource, Sari, Iran
2 Industrial wood and paper Department, University of Agricultural Sciences and Natural Resource, Sari, Iran
3 Pharmaceutical Chemistry Department, Mazandaran University of Medical Science, Sari, Iran
چکیده [English]

The purpose of this study is to evaluate total phenol and flavonoids and antioxidant effects of beech and hornbeam and spruce; bark. Beech and hornbeam species of northern hardwood and spruce; species of planting trees, despite of their abundance, very little information about the chemical composition of their skin and their application is presented. Antioxidant compounds that prevent disease outbreaks and destruction of food can also be extracted from the skin these plants. After preparation of the bark, acetone extracts were obtained by soxhlet. First, the amount of total phenol and flavonoid extracts measured and then for evaluating the antioxidant properties of extracts; regenerative power and hydrazyl diphenyl pykryl methods were used. The results showed that the amount of phenol and flavonoids were highest in the bark of hornbeam and lowest in the bark of beech. Test results or trapping free radicals DPPH1 shpwed inhibitory concentrations 50% acetone extract of the bark of beech, hornbeam and spruce; respectively, 07.63, 17.33 and 3.86 micrograms per milliliter, respectively. Also beech extracts has more regeneration power compare with other species.
 

کلیدواژه‌ها [English]

  • Antioxidant activity
  • beach
  • hornbeam
  • populous
  • total phenol

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